By John N. Abelson, Melvin I. Simon, Sidney Fleischer, Becca Fleischer
The delivery volumes of the Biomembranes sequence have been initiated with Volumes one hundred twenty five and 126 of tools in Enzymology, which coated shipping in micro organism, Mitochondria, and Chloroplasts. Volumes 156 and 157 persevered the subject matter with ATP-Driven Pumps and comparable shipping. mobile and Subcellular delivery: Eukaryotic (Nonepithelial) Cells used to be the subject of Volumes 173 and 174. The subject matter of this quantity, in addition to of quantity 192, is mobile and Subcellular delivery: Epithelial Cells
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Additional info for Biomembranes Part V: Cellular and Subcellular Transport: Epithelial Cells
9) ion flux mechanisms in parallel with the Na + pump; however, the concept was later reinterpreted by Lewis and collaborators,~° who also derived Eq. 2°,2~,35-39 Ra was either increased by increasing concentrations of amiloride or decreased by ionophores such as amphotericin B, gramicidin D, nystatin, novobiocin, silver ions, or by stimulation with vasopressin. Comment. The method is subject to the general criticism of the underlying model circuit (Fig. lb; see Introduction). In addition, its application is restricted to epithelia whose properties are already largely known.
Physiol. 232, F448 (1977). 37 W. S. Marshall and S. D. Klycc, J. Membr. Biol. 73, 275 (1983). 3s N. K. Wills and C. Clauscn, J. Membr. Biol. 95, 21 (1987). 39 R. Rick, A. D6rgc, and E. Sessclmann, PfluegersArch. 411, 243 (1988).  PARACELLULAR SHUNT CONDUCTANCE IN EPITHELIA 15 In Appendix B we report some model calculations which define the validity limits of the method quantitatively. From this we deduce that the method is restricted to the simple model of tight Na+-absorbing epithelia in which R , is exclusively selective for Na + and to the use of ionophores that are also purely selective for Na +.
It also allows changes in cell membrane capacitances to be followed that might occur in response to neurovegetative stimuli (insertion/removal of proteins and lipids into the cell membranes). The method has the following drawbacks: (1) From a technical point of view it is extremely involved; (2) due to the specific distribution of cell membrane and shunt resistances in leaky epithelia, the estimated cell membrane resistances exhibit large scatter so that the measurements have 52 p. Weskamp, Pfluegers Arch.