By Joost van Mameren, Gijs J. L. Wuite, Iddo Heller (auth.), Erwin J. G. Peterman, Gijs J. L. Wuite (eds.)
Life scientists think that lifestyles is pushed, directed, and formed by way of biomolecules engaged on their very own or in live performance. it's only within the previous couple of a long time that technological breakthroughs in delicate fluorescence microscopy and single-molecule manipulation strategies have made it attainable to monitor and control unmarried biomolecules and degree their person houses. The methodologies provided in Single Molecule strategies: tools and Protocols are being utilized a growing number of to the examine of biologically appropriate molecules, similar to DNA, DNA-binding proteins, and motor proteins, and have gotten regular in molecular biophysics, biochemistry, and molecular and mobile biology. the purpose of Single Molecule ideas: tools and Protocols is to supply a wide evaluate of single-molecule ways utilized to biomolecules at the foundation of transparent and concise protocols, together with a superb advent to the main primary single-molecule concepts, akin to optical tweezers, single-molecule fluorescence instruments, atomic strength microscopy, magnetic tweezers, and tethered particle movement. Written within the hugely profitable Methods in Molecular Biology™ sequence structure, chapters include introductions to their respective subject matters, lists of the mandatory fabrics and reagents, step by step, effortlessly reproducible laboratory protocols, and notes on troubleshooting and keeping off identified pitfalls.
Authoritative and available, Single Molecule ideas: tools and Protocols serves as an excellent advisor to scientists of all backgrounds and gives a extensive and thorough evaluation of the intriguing and still-emerging box of single-molecule biology.