By H. Mori, N. Nakagawa, T. Ono, N. Yamagishi, H. Imaseki (auth.), Jean Claude Pech, Alain Latché, Claudine Balagué (eds.)
The foreign Symposium on "Cellular and Molecular elements of Biosynthesis and motion of the Plant Hormone Ethylenc" ,vas held in Agen, France from August 31 st and September 4th, 1992. The making plans and administration of the clinical and social programme of the convention have been performed together by means of the "Ethylene study team" of ENSAlIN"P (Toulouse) and Agropole Congres carrier (Agen). because the final conferences in Israel (1984) and in Belgium (1988), ethylene body structure has undergone a interval of fascinating development as a result of new advancements in mobile and molecular bioiogy. New tools and instruments were built to raised comprehend the position and features of ethylene in fruit ripening, flower senescence, abscission, piant progress, and telephone differentiation. Genes thinking about ethylene biosynthesis were characterised and transgenic vegetation with altered ethylene construction were generated. The feasibility of delaying fruit ripening or flower senescence through genetic manipulation is now verified, therefore beginning new views for the postharvest dealing with of plant items. a few growth has additionally been made at the realizing of ethylene motion. besides the fact that, a lot continues to be performed during this quarter to explain the ethylene sign transduction pathway. round one hundred forty scientists from 20 nations attended the Symposium. They awarded forty seven oral experiences and forty poster demonstrations. them all are released in those lawsuits. it's been a excitement for us to prepare this crucial Symposium and to edit this book.
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Extra info for Cellular and Molecular Aspects of the Plant Hormone Ethylene: Proceedings of the International Symposium on Cellular and Molecular Aspects of Biosynthesis and Action of the Plant Hormone Ethylene, Agen, France, August 31–September 4, 1992
1986) Use of monoclonal antibodies in the purification and characterization of l-aminocyclop~opane-l carboxylate synthase, an enzyme in ethylene biosynthesis. Proc. Natl. Acad. Sci. USA 83, 7755-7759. 7. Satoh, S. F. (1989) Specificity of S-adenosyl-L-methionine in the inactivation and the labeling of l-aminocyclo-propane-l-carboxylate synthase isolated from tomato fruits. Plant Physiol. Arch. Biochem. Biophys. 271, 107-112. 8. Theo10gis, A. (1992) One rotten apple spoils the whole bushel: the role of ethylene in 9.
1988) S-Adenosylmethionine-dependent inactivation and radiolabeling of l-aminocyclopropane-l-carboxylate synthase isolated from tomato fruits. Plant Physiol. 88, 109-114. 6. c. and Kende, H. (1986) Use of monoclonal antibodies in the purification and characterization of l-aminocyclop~opane-l carboxylate synthase, an enzyme in ethylene biosynthesis. Proc. Natl. Acad. Sci. USA 83, 7755-7759. 7. Satoh, S. F. (1989) Specificity of S-adenosyl-L-methionine in the inactivation and the labeling of l-aminocyclo-propane-l-carboxylate synthase isolated from tomato fruits.
The homogenate was filtered through a double layer of 33 J. C. ), Cellular and Molecular Aspects of the Plant Hormone Ethylene, 33-38. © 1993 Kluwer Academic Publishers. 34 muslin before centrifugation at 21,000 g for 20 min. Aliquots of the extract were then frozen at -70°C until used as the enzyme extract. When apple was used, the enzyme was prepared as for melon, except that 100 g of ground pulp was homogenised with sand in 200 ml of extraction buffer, which was supplemented with 5% (w/v) PVPP  and 30 mM sodium ascorbate.