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By Bernard Leonard Horecker; Earl Reece Stadtman

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Dx (113). The pH optima are dependent on the substrate. 0 (50). 0 (117). B. Protein Kinase Substrates Although cGMP is considered to exert specific metabolic regulatory effects by phosphorylation of protein substrates distinct from those phosphorylated by A-kinase, such substrates have not been unequivo­ cally demonstrated. In experiments in which [γ- 32 Ρ]ΑΤΡ and cGMP were incubated with cellular fractions, phosphorylation of specific pro­ tein was observed on electrophoretic separation (118-120).

And Fain, J. , J. Biol. Chem. 251, 2987 (1976). 131. Corbin, J. , Kelly, S. , and Park, C. , J. Biol. Chem. 250, 218 (1975). 132. Donnelly, T. , Kuo, J. F , Reyes, P. , J. Biol. Chem. 248, 190 (1973). 133. Donnelly, T. , Kuo, J. , and Greengard, P , J. Biol. Chem. 248, 199 (1973). 134. , and Kuo, J. , J. Biol. Chem. 251, 4283 (1976). 135. Kuo, J. E, Malveaux, E. , Patrick, J. , Davis, C. , and Pruitt, A. , Biochim. Biophys. Acta 497, 785 (1977). 136. Walsh, D. , Ashby, C. , Fischer, E. , and Krebs, E.

Cyclic Nucleotide Res. 2, 71 (1976). 130. Pointer, R. , and Fain, J. , J. Biol. Chem. 251, 2987 (1976). 131. Corbin, J. , Kelly, S. , and Park, C. , J. Biol. Chem. 250, 218 (1975). 132. Donnelly, T. , Kuo, J. F , Reyes, P. , J. Biol. Chem. 248, 190 (1973). 133. Donnelly, T. , Kuo, J. , and Greengard, P , J. Biol. Chem. 248, 199 (1973). 134. , and Kuo, J. , J. Biol. Chem. 251, 4283 (1976). 135. Kuo, J. E, Malveaux, E. , Patrick, J. , Davis, C. , and Pruitt, A. , Biochim. Biophys. Acta 497, 785 (1977).

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